Session U24 - Biological Materials on Solids II: DMP Focused Session.
MIXED session, Thursday morning, March 19
501C, Los Angeles Convention Center
Surfaced-based DNA computation allows for the efficient manipulation of operations on DNA strands. The readout operation determines the DNA strand sequence that encodes the solution of a combinatorial problem of interest; to perform it, densely addressed arrays are a necessity. In our surfaced-based approach, we photopattern self-assembled monolayers (SAMs) attached to a gold surface creating specific regions of hydrophilic islands in a hydrophobic background, and we characterize the chemically modified surface through reflection FTIR and fluorometry. Subsequently, the DNA strands, short 31 base-pair oligonucleotides that encode 4-8 bits of data, attach to the hydrophilic islands and form addressed arrays with feature sizes in the submillimeter range. With simple addressed arrays, we can perform the readout operation for a combinatorial problem. Expanding this simple technique, possibly with ink jet printer technology, readout can be modified to solve complex combinatorial problems employing arrays of 16 by 16 or larger with features sizes on the micrometer scale.