Rapid and quantitative detection of the microbial spoilage of beef by Fourier transform infrared spectroscopy and machine learning

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@Article{Ellis:2004:ACA,

• author = "David I. Ellis and David Broadhurst and Royston Goodacre",
• title = "Rapid and quantitative detection of the microbial spoilage of beef by Fourier transform infrared spectroscopy and machine learning",
• journal = "Analytica Chimica Acta",
• year = "2004",
• volume = "514",
• number = "2",
• pages = "193--201",
• month = "1 " # jul,
• keywords = "genetic algorithms, genetic programming, Muscle foods, FT-IR spectroscopy, Food spoilage, Chemometrics, Evolutionary computation",
• ISSN = "0003-2670",
• owner = "wlangdon",
• broken = "http://www.sciencedirect.com/science/article/B6TF4-4CDJJ78-5/2/63df147cb89407ac7ac8bf9d093580f7",
• URL = "http://dbkgroup.org/dave_files/ACAbeef04.pdf",
• DOI = "doi:10.1016/j.aca.2004.03.060",
• abstract = "Beef is a commercially important and widely consumed muscle food and central to the protein intake of many societies. In the food industry no technology exists for the rapid and accurate detection of microbiologically spoiled or contaminated beef. Fourier transform infrared (FT-IR) spectroscopy is a rapid, reagentless and non-destructive analytical technique whose continued development is resulting in manifold applications across a wide range of biosciences. FT-IR was exploited to measure biochemical changes within the fresh beef substrate, enhancing and accelerating the detection of microbial spoilage. Separately packaged fresh beef rump steaks were purchased from a national retailer, comminuted for 15 s and left to spoil at ambient room temperature for 24 h. Every hour, FT-IR measurements were collected directly from the sample surface using attenuated total reflectance, in parallel the total viable counts of bacteria were obtained by classical microbiological plating methods. Quantitative interpretation of FT-IR spectra was undertaken using partial least squares regression and allowed for accurate estimates of bacterial loads to be calculated directly from the meat surface in 60 s. Machine learning methods in the form of genetic algorithms and genetic programming were used to elucidate the wavenumbers of interest related to the spoilage process. The results obtained demonstrated that using FT-IR and machine learning it was possible to detect bacterial spoilage rapidly in beef and that the most significant functional groups selected could be directly correlated to the spoilage process which arose from proteolysis, resulting in changes in the levels of amides and amines.",

}

Genetic Programming entries for David I Ellis David I Broadhurst Royston Goodacre

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